i-Cyt Reflection
The i-Cyt Reflection cell sorter and analyzer provides modern advances with proven technology. This is the first cell sorter that allows for optical flexibility of up to 48 PMTs and 256 parameters, maximum performance potential with up to four HAPS (Highly Automated Parallel Sorters) in one unit that can run in unison or individually, remote monitoring capabilities, BSL-2 safety features and sharing of resources to help with economical constraints (sharing of lasers, PMTs, and computers).
Currently our instrument features a dual HAPS system (two sorters). The instrument is equipped with a shared 500mW, 488nm laser that can detect colors at 525/40, 575/40, 645/20, 670/30 and two 30mw-635nm lasers that can detect colors at 720/50 and 780/60. Each module is also equipped with a FSC and BSC PMT as well. Additional colors can be accomodated through the use of interchangeable filters that are featured on this instrument, however pre-consultaion with the operator is required. This instrument utilizes WinList software for analysis and data acquisition.
The Reflection Cell Sorter is able to sort from a microcentrifuge tube or a 12 X 75mm falcon tube into, multiple formats including, up to four microfuge tubes, four 12 X 75mm tubes, two 15mL conical tubes, or one 50mL conical tube. This is instrument is equipped with a plate sorter that can accomodate 6-396 well formats.
This instrument is supported through the generous donations of The Comprehensive Cancer Center, The Department of Veterinary Biosciences and The OSU Retrovirus Group. For further information please contact Nicole White at 292-9716.
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