The latest tool for AFM in biological applications, the Bioscope II with Nanoscope V controller (Digital Instruments, Santa Barbara, CA), is available here. The state-of-the-art instrument can perform simultaneous AFM and light/fluorescence microscopy on biological samples, force sensing at nanonewton scales, magnetic force imaging, nanoindentations - to name just a few applications. Aditionally, the AFM core facility is equipped with a high resolution Veeco Multimode AFM Microscope.
We train and assist users in use of AFM for their research, consult and advise users on experimental design, offer equipment time or perform AFM experiments and related data analysis for them. Please contact AFM core manager Daniel Iscru for further details.
Images below are a few representative images obtained in the AFM core lab by staff or by users trained in-house.
Recent publications
Iscru, D.F., Anghelina, M., Agarwal, S., Agarwal, G., Changes in surface topologies of chondrocytes subjected to mechanical forces: an AFM analysis, Journal of Structural Biology (2008), doi: 10.1016/j.jsb.2008.02.005
Mayur Savla, Sharon Schreiber, Denis V. Pelekhov, Daniel F. Iscru, Camelia Selcu, P. Chris Hammel and Gunjan Agarwal. Magnetic Force Microscopy of Superparamagnetic Nanoparticles, Small (2008) 4(2):270-278.
Gunjan Agarwal, Cosmin Mihai and Daniel F. Iscru. Interaction of Discoidin Domain Receptor 1 with Collagen type 1. J. Mol. Biol. (2007) 367, 443-455.
Hui Shao, Jeffrey W. Lockman, and Jon R. Parquette Coupled conformational equilibria in beta-sheet peptide-dendron conjugates. J Am Chem Soc. 2007 Feb 21;129(7):1884-5. Epub 2007 Jan
Cosmin Mihai, Daniel F. Iscru, Lawrence J. Druhan, Terry S. Elton and Gunjan Agarwal. Discoidin Domain Receptor 2 Inhibits Fibrillogenesis of Collagen Type 1. J. Mol. Biol. (2006) 361, 864-876.
